Biotin (Vitamin H) ELISA

Parameter Cat. No.
Biotin ELISA BIO-E01  

Intended Use:

The Immunolab Biotin ELISA is a sensitive method for the quantitative determination
of biotin (Vitamin H) in multivitamin tablets, -juices, -jam, -sweets, grain products (muesli)
and isotonic drink powders.


Test principle: Competitive enzyme immunoassay
Format: Microtiter plate with 96 wells
Incubation time: 90 min (60/30 min)
Standards: 0; 1; 2.5; 5; 10; 25 [ng/mL]
Detection: 450 nm (HRP + TMB)
Detection limit:

Depends on the sample preparation


Biotin-4-aminobenzoate     80%
Biocytin    71%

Storage: 2–8°C